Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2011 Dec 19;208(13):2563-4.
doi: 10.1084/jem.20112232.

A human equivalent of mouse B-1 cells?

Marc Descatoire, Jean-Claude Weill, Claude-Agnès Reynaud, Sandra Weller
Comment

A human equivalent of mouse B-1 cells?

Marc Descatoire et al. J Exp Med. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
CD3, CD43, CD38 and IgD expression on human CD20+CD27+ PMBCs. (A) Human PMBCs were analyzed within a large lymphoid gate. The right dot plot represents cells after enrichment by CD19 microbeads (Miltenyi Biotec), whereas the left dot plot represents total PMBCs. The flow cytometric analysis shown was performed at a flow rate of <5,000 events per second. The data shown here for an adult are representative of eight adult blood samples analyzed in four separate experiments. (B) Representative analysis of an adult blood sample. PBMCs were analyzed within a large lymphoid gate, with doublets excluded by SSC-W criteria. Cells were further gated as CD3−CD20+ for analysis of CD27 and CD43 expression. The small top right gate in the dot plot indicates plasmablasts that are excluded from the cell estimates. The histograms show the expression of IgD and CD38 on the CD43+CD27+ population. (C) Blood samples from 6 children (3–6.5 yr old) and 8 adults were analyzed as in B. The proportion of CD43+CD27+ cells among CD3−CD20+ B cells (expressed either as percentage of total B cells or of CD27+ B cells) is indicated. Each bar represents the results obtained for one individual. (D) The relative distribution of IgD+ and IgD− B cells among the CD27+CD43+ B cell population is shown in bar graphs, each bar representing the results obtained for one individual. The following antibodies were used: CD20 (APC-H7, clone 2H7), CD43 (FITC, clone 1G10), CD3 (PE, clone UCHT1), and CD27 (APC, clone M-T271) from BD; CD38 (PerCp-Cy5.5, clone HIT2) and IgD (PE-Cy7, clone IAG-2) from BioLegend. Flow cytometry analysis was performed on a FACSCanto II apparatus with FACSDiva software.
See this image and copyright information in PMC

Comment in

Comment on

References

    1. Baumgarth N. 2011. The double life of a B-1 cell: self-reactivity selects for protective effector functions. Nat. Rev. Immunol. 11:34–46. 10.1038/nri2901 - DOI - PubMed
    1. Boumsell L., Bernard A., Lepage V., Degos L., Lemerle J., Dausset J.. 1978. Some chronic lymphocytic leukemia cells bearing surface immunoglobulins share determinants with T cells. Eur. J. Immunol. 8:900–904. 10.1002/eji.1830081214 - DOI - PubMed
    1. Cuss A.K., Avery D.T., Cannons J.L., Yu L.J., Nichols K.E., Shaw P.J., Tangye S.G.. 2006. Expansion of functionally immature transitional B cells is associated with human-immunodeficient states characterized by impaired humoral immunity. J. Immunol. 176:1506–1516. - PubMed
    1. Good K.L., Avery D.T., Tangye S.G.. 2009. Resting human memory B cells are intrinsically programmed for enhanced survival and responsiveness to diverse stimuli compared to naive B cells. J. Immunol. 182:890–901. - PubMed
    1. Griffin D.O., Holodick N.E., Rothstein T.L.. 2011. Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−. J. Exp. Med. 208:67–80. 10.1084/jem.20101499 - DOI - PMC - PubMed