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Comment
. 2011 Dec 19;208(13):2566-9.
doi: 10.1084/jem.20111761.

Human B1 cells are CD3-: A reply to "A human equivalent of mouse B-1 cells?" and "The nature of circulating CD27+CD43+ B cells"

Daniel O Griffin  1 , Nichol E Holodick, Thomas L Rothstein
Affiliations
Comment

Human B1 cells are CD3-: A reply to "A human equivalent of mouse B-1 cells?" and "The nature of circulating CD27+CD43+ B cells"

Daniel O Griffin et al. J Exp Med. .
No abstract available

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Figures

Figure 1.
Figure 1.
Human CD20+CD27+CD43+ B1 cells do not express CD3. (A) Adult peripheral blood cells were immunofluorescently stained with specific antibodies. CD20+CD27+CD43+ B1 cells were sort-purified from nonenriched, as well as CD19-enriched PBMCs and sorted B1 cells were analyzed for CD3 expression. Gating was based on an isotype control. Results for one of three comparable experiments are shown. (B) Adult peripheral blood cells were immunofluorescently stained, sort-purified for naive (CD19+CD27−CD43−/CD20+CD27−CD43−) and B1 (CD19+CD27+CD43+/CD20+CD27+CD43+) cells, and examined by confocal microscopy. (C) Immunofluorescently stained PBMCs were analyzed at high concentration without vortexing without (top row) or with (bottom row) FSC-H/FSC-A doublet discrimination. The last panel shows CD3+ events among CD20+CD27+CD43+ cells. (D) The same sample as in C was diluted and vortexed, and then analyzed without (top row) or with (bottom row) FSC-H/FSC-A doublet discrimination. Note that many more cells appear as singlets (99.1%) as compared with C (75.9%). The last panel shows CD3+ events among CD20+CD27+CD43+ cells. Results for one of three comparable experiments are shown. (E–G) Adult peripheral blood cells were immunofluorescently stained, and then presented for flow cytometric analysis at various cell concentrations, as indicated (n = 12). The proportion of single cells determined by FSC-H/FSC-A gating is shown as a function of cell concentration in E. The proportion of CD3+ events among gated CD20+CD27+ cells is shown as a function of cell concentration in F. The proportion of CD3+ events among gated CD20+CD27+CD43+ cells is shown as a function of cell concentration in G. (H) Adult peripheral blood cells were immunofluorescently stained with specific antibodies, brought into a single cell suspension, and sort-purified on an Influx (BD) instrument. The gating strategy used for experiments reported in Griffin et al. (2011) is shown.
See this image and copyright information in PMC

Comment on

  • A human equivalent of mouse B-1 cells?
    Descatoire M, Weill JC, Reynaud CA, Weller S. Descatoire M, et al. J Exp Med. 2011 Dec 19;208(13):2563-4. doi: 10.1084/jem.20112232. J Exp Med. 2011. PMID: 22184680 Free PMC article. No abstract available.
  • The nature of circulating CD27+CD43+ B cells.
    Perez-Andres M, Grosserichter-Wagener C, Teodosio C, van Dongen JJ, Orfao A, van Zelm MC. Perez-Andres M, et al. J Exp Med. 2011 Dec 19;208(13):2565-6. doi: 10.1084/jem.20112203. J Exp Med. 2011. PMID: 22184681 Free PMC article. No abstract available.

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