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. 2011:2011:650979.
doi: 10.1155/2011/650979. Epub 2011 Dec 10.

Photo-irradiated biosynthesis of silver nanoparticles using edible mushroom pleurotus Florida and their antibacterial activity studies

Ravishankar Bhat  1 , Raghunandan Deshpande, Sharanabasava V Ganachari, Do Sung Huh, A Venkataraman
Affiliations

Photo-irradiated biosynthesis of silver nanoparticles using edible mushroom pleurotus Florida and their antibacterial activity studies

Ravishankar Bhat et al. Bioinorg Chem Appl. 2011.

Abstract

This is a report on photo-irradiated extracellular synthesis of silver nanoparticles using the aqueous extract of edible oyster mushroom (Pleurotus florida) as a reducing agent. The appearance, size, and shape of the silver nanoparticles are understood by UV-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The X-ray diffraction studies, energy dispersive X-ray analysis indicate that particles are crystalline in nature. Fourier transform infrared spectroscopy analysis revealed that the nanoparticles are covered with biomoieties on their surface. As can be seen from our studies, the biofunctionalized silver nanoparticles thus produced have shown admirable antimicrobial effects, and the synthetic procedure involved is eco-friendly and simple, and hence high range production of the same can be considered for using them in many pharmaceutical applications.

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Figures

Figure 1
Figure 1
Image of mushroom Pleurotus florida.
Figure 2
Figure 2
UV-vis spectra indicating the photo-irradiated AgNP synthesis recorded as a function of time. The change in color of the reaction mixture and its respective surface plasmon resonance is shown with the lines.
Figure 3
Figure 3
XRD pattern of crystalline AgNPs.
Figure 4
Figure 4
Energy dispersive X-ray spectrum (EDAX) of metallic AgNP.
Figure 5
Figure 5
AFM images of biofunctionalized AgNPs embedded in organic moiety.
Figure 6
Figure 6
TEM images of AgNP.
Figure 7
Figure 7
FTIR spectrum of AgNP.
Figure 8
Figure 8
Probable pathway of synthesis mechanism.
Figure 9
Figure 9
Antimicrobial activity of the AgNP using edible mushroom Pleurotus florida. Effects of AgNP with cefixime, AgNP, and the standard drug silver sulfadiazine (silzin) in different concentrations are shown in the figure.
See this image and copyright information in PMC

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