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. 2012;27(1):99-104.
doi: 10.1264/jsme2.me11270. Epub 2011 Dec 29.

Actinobacteria associated with the marine sponges Cinachyra sp., Petrosia sp., and Ulosa sp. and their culturability

Shams Tabrez Khan  1 , Motoki Takagi, Kazuo Shin-ya
Affiliations

Actinobacteria associated with the marine sponges Cinachyra sp., Petrosia sp., and Ulosa sp. and their culturability

Shams Tabrez Khan et al. Microbes Environ. 2012.

Abstract

Actinobacteria associated with 3 marine sponges, Cinachyra sp., Petrosia sp., and Ulosa sp., were investigated. Analyses of 16S rRNA gene clone libraries revealed that actinobacterial diversity varied greatly and that Ulosa sp. was most diverse, while Cinachyra sp. was least diverse. Culture-based approaches failed to isolate actinobacteria from Petrosia sp. or Ulosa sp., but strains belonging to 10 different genera and 3 novel species were isolated from Cinachyra sp.

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Figures

Fig. 1
Fig. 1
Phylogenetic dendrogram of representative actinobacteria clones from Ulosa sp., Petrosia sp., and Cinachyra sp. Related sequences were downloaded from the DNA Databank of Japan (DDBJ). The tree was calculated with a neighbor-joining algorithm and bootstrapped using 1000 replicates. Only those bootstrap values that exceeded 75% are shown.
See this image and copyright information in PMC

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