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. 2012 Apr;113(4):442-4.
doi: 10.1016/j.jbiosc.2011.11.019. Epub 2012 Jan 3.

Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

Jianlong He  1 , Kouta Sakaguchi, Tohru Suzuki
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Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

Jianlong He et al. J Biosci Bioeng. 2012 Apr.

Abstract

The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression.

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