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. 2012 Jan 27;51(5):1216-20.
doi: 10.1002/anie.201105882. Epub 2011 Dec 23.

Enzymatic total synthesis of defucogilvocarcin M and its implications for gilvocarcin biosynthesis

Pallab Pahari  1 , Madan K Kharel, Micah D Shepherd, Steven G van Lanen, Jürgen Rohr
Affiliations

Enzymatic total synthesis of defucogilvocarcin M and its implications for gilvocarcin biosynthesis

Pallab Pahari et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
HPLC traces of the enzymatic reactions for the synthesis of defucogilvocarcin M (1): A) Control reaction without acetyl-CoA and malonyl-CoA. B) Acetyl-CoA+malonyl-CoA+PKS enzymes. C) Acetyl-CoA + malonyl-CoA + PKS enzymes + post-PKS enzymes. D) Rabelomycin (12) standard. E) Defucogilvocarcin M (1) standard.
Figure 2
Figure 2
HPLC traces of the enzymatic reactions with prejadomycin (14) and UWM6 (10): A) Prejadomycin (14) + PKS-enzymes + post-PKS enzymes. B) Prejadomycin (14) standard. C) UWM6 (10) + PKS-enzymes + post-PKS enzymes. D) UWM6 (10) standard. E) Defucogilvocarcin M (1) standard.
Figure 3
Figure 3
HPLC traces of the enzymatic reactions: A) Acetyl CoA + malonyl-CoA + PKS enzymes + JadF produce prejadomycin (14). B) Prejadomycin (14) + GilOI. C) Dehydrorabelomycin (16) standard. D) Dehydrorabelomycin (16) + GilOII + GilM + GilMT + GilR produce defucogilvocarcin M 1.
Scheme 1
Scheme 1
Representative members of the gilvocarcin group of natural products.
Scheme 2
Scheme 2
Proposed gilvocarcin biosynthetic pathway.
See this image and copyright information in PMC

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