Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct 1;271(1):281-4.
doi: 10.1042/bj2710281.

Evidence by chemical modification that tryptophan-104 of the cysteine-proteinase inhibitor chicken cystatin is located in or near the proteinase-binding site

M Nycander  1 , I Björk
Affiliations

Evidence by chemical modification that tryptophan-104 of the cysteine-proteinase inhibitor chicken cystatin is located in or near the proteinase-binding site

M Nycander et al. Biochem J. .

Abstract

The single tryptophan residue Trp-104 of chicken cystatin was modified with a 2-hydroxy-5-nitrobenzyl group. The change of the absorption spectrum of this group on binding of the modified cystatin to papain indicated a decreased environmental polarity of the probe. The modified inhibitor had about a 10(5)-fold lower affinity for papain than had intact cystatin, this being due to a higher dissociation rate constant. These results show that Trp-104 of cystatin is located in or near the proteinase-binding site of the inhibitor, in agreement with a model proposed from computer docking Experiments.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 1970 May 10;245(9):2247-54 - PubMed
    1. J Am Chem Soc. 1965 Mar 5;87:1126-32 - PubMed
    1. Arch Biochem Biophys. 1974 Sep;164(1):30-6 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Biochemistry. 1978 Aug 8;17(16):3339-44 - PubMed

Publication types