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. 2012 Feb;29(2):175-83.
doi: 10.1007/s10815-011-9706-6. Epub 2012 Jan 10.

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Ali Talebi  1 , Saeed Zavareh, Maryam Hajighasem Kashani, Taghi Lashgarbluki, Isaac Karimi
Affiliations

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Ali Talebi et al. J Assist Reprod Genet. 2012 Feb.

Abstract

Purpose: This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue.

Methods: Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α- minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively.

Results: In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p < 0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p < 0.05).

Conclusion: ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.‎‎‎

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Figures

Fig. 1
Fig. 1
Representative photographs of mouse preantral follicles (from 14-day old mouse) cultured in vitro for 12 days. The cultured isolated follicle on day 2 (a), day 4 (b), day 6 (c), day 8 (d), day 10 (e) and The oocyte ovulated of cultured preantral follicle after hCG administration (f). Antral-like cavities are indicated by black arrow
Fig. 2
Fig. 2
ROS concentrations in cultured preantral follicles in the presence of different concentration of ALA. In all cases 4 experimental replicates were performed. Groups with different superscripts (a, b, c, d, e) are significantly different (p < 0.05) at same times of culture within the different groups. Different superscripts (A, B, C, D) describe significantly different (p < 0.05) at different times of culture within the same group. ROS: Reactive Oxygen Species; ALA: Alpha lipoic acid
Fig. 3
Fig. 3
TAC levels in cultured preantral follicles in the presence of different concentration of ALA. In all cases 4 experimental replicates were performed. Groups with different superscripts (a, b, c, d, e) are significantly different (p < 0.05) at same times of culture within the different groups. Different superscripts (A, B, C, D) describe significantly different (p < 0.05) at different times of culture within the same group. TAC: Total Antioxidant capacity; ALA: Alpha lipoic acid
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