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. 2012;76(1):180-2.
doi: 10.1271/bbb.110528. Epub 2012 Jan 7.

Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome

Mitsuhiro Itaya  1
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Free article

Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome

Mitsuhiro Itaya. Biosci Biotechnol Biochem. 2012.
Free article

Abstract

Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.

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