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. 2012 Jan;86(1):159-65.
doi: 10.4269/ajtmh.2012.11-0475.

Virological surveillance of dengue in Saint Martin and Saint Barthelemy, French West Indies, using blood samples on filter paper

Séverine Matheus  1 , Jean-Loup Chappert, Sylvie Cassadou, Franck Berger, Bhetty Labeau, Laetitia Bremand, Alain Winicki, Patricia Huc-Anais, Philippe Quenel, Philippe Dussart
Affiliations

Virological surveillance of dengue in Saint Martin and Saint Barthelemy, French West Indies, using blood samples on filter paper

Séverine Matheus et al. Am J Trop Med Hyg. 2012 Jan.

Abstract

To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes.

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Figures

Figure 1.
Figure 1.
Weekly number of biologically confirmed cases of dengue and weekly distribution of serotype, Saint Martin, Epi-week 2008-39–2010-52.
Figure 2.
Figure 2.
Weekly number of biologically confirmed cases of dengue and weekly distribution of serotype, Saint Barthélemy, Epi-week 2008-39–2010-52.
See this image and copyright information in PMC

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