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. 2011 Dec;27(4):365-8.
doi: 10.5625/lar.2011.27.4.365. Epub 2011 Dec 19.

Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus

Sang-Chul Kwon  1 , Yun-Bae Kim
Affiliations

Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus

Sang-Chul Kwon et al. Lab Anim Res. 2011 Dec.

Abstract

Antioxidative and aldose reductase (AR)-inhibitory effects of a fermentation filtrate of Rubus coreanus (FRC) were investigated using corneal/retinal homogenate and lens cytosol, respectively. Rat corneal/retinal homogenate was treated with 50 µM FeCl(3) in the presence of FRC (3.2-100 µg/mL) for 30 min at 37℃, and thiobarbituric acid-reactive substances (TBARS) was quantified as a lipid peroxidation parameter. FRC markedly suppressed the TBARS production in a concentration-dependent manner, leading to 50% (IC(50)) and 100% (IC(100)) inhibitory concentrations of 20 and 95 µg/mL, respectively, which was similar to the effect of butylated hydroxyanisole. Activity of AR from rat lens was assayed in the presence of FRC (1-31.6 µg/mL) at 25℃ using glyceraldehyde as a substrate. FRC inhibited lens AR by 50% (IC(50)) and 90% (IC(90)) at approximately 2 and 31.6 µg/mL, respectively, comparable to the effect of quercetin. The results indicate that ERC could be a promising candidate for the improvement of eye injury and visual dysfunction of dry eye and diabetic patients.

Keywords: Rubus coreanus; aldose reductase inhibition; antioxidative activity.

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References

    1. Nakamura S, Shibuya M, Nakashima H, Imagawa T, Uehara M, Tsubota K. D-β-Hydroxybutyrate protects against corneal epithelial disorders in a rat dry eye model with jogging board. Invest Ophthalmol Vis Sci. 2005;46:2379–2387. - PubMed
    1. Holly FJ, Lemp MA. Tear physiology and dry eyes. Surv Ophthalmol. 1977;22:69–87. - PubMed
    1. Tseng SC, Tsubota K. Important concepts for treating ocular surface and tear disorders. Am J Ophthalmol. 1997;124:825–835. - PubMed
    1. Nakamura S, Shibuya M, Nakashima H, Hisamura R, Masuda N, Imagawa T, Uehara M, Tsubota K. Involvement of oxidative stress on corneal epithelial alterations in a blink-suppressed dry eye. Invest Ophthalmol Vis Sci. 2007;48:1552–1558. - PubMed
    1. Tsubota K, Nakamori K. Dry eyes and video display terminals. N Engl J Med. 1993;328:584. - PubMed