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. 1990 Nov 9;1036(2):162-5.
doi: 10.1016/0304-4165(90)90029-v.

Isolation and characterization of a new lectin from plasma of fish Channa punctatus

S R Manihar  1 , H R Das
Affiliations

Isolation and characterization of a new lectin from plasma of fish Channa punctatus

S R Manihar et al. Biochim Biophys Acta. .

Abstract

A soluble lectin is purified to apparent homogeneity from plasma of Channa punctatus by affinity chromatography on N-acetyl-D-galactosamine coupled to epoxy-activated cellulose. The lectin has 140 kDa native molecular mass and 68 kDa subunit molecular mass, as determined by native and sodium dodecyl sulphate denaturing polyacrylamide gel electrophoresis, respectively. The lectin agglutinates human A and AB blood groups and rat, mice and guinea pig erythrocytes in the presence of Ca2+ and Mg2+ or Mn2+ ions. These divalent cations, but not thiol group, are obligatory requirements for the lectin activity. Gal(beta 1----3)GalNAc (0.09 mM) is the most potent inhibitor of the lectin.

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