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Comparative Study
. 2012 Apr;50(4):1437-9.
doi: 10.1128/JCM.06746-11. Epub 2012 Jan 11.

Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci

Anne J M Loonen  1 , Arjan R Jansz, Jeandery N B Bergland, Marion Valkenburg, Petra F G Wolffs, Adriaan J C van den Brule
Affiliations
Comparative Study

Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci

Anne J M Loonen et al. J Clin Microbiol. 2012 Apr.

Abstract

Five methods were compared to determine the most accurate method for identification of coagulase-negative staphylococci (CoNS) (n = 142 strains). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) showed the best results for rapid and accurate CoNS differentiation (99.3% of strains correctly identified). An alternative to this approach could be Vitek2 combined with partial tuf gene sequencing (100% of strains correctly identified when both methods are performed simultaneously).

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