Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2012 Apr;50(4):1437-9.
doi: 10.1128/JCM.06746-11. Epub 2012 Jan 11.

Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci

Anne J M Loonen  1 , Arjan R Jansz, Jeandery N B Bergland, Marion Valkenburg, Petra F G Wolffs, Adriaan J C van den Brule
Affiliations
Comparative Study

Comparative study using phenotypic, genotypic, and proteomics methods for identification of coagulase-negative staphylococci

Anne J M Loonen et al. J Clin Microbiol. 2012 Apr.

Abstract

Five methods were compared to determine the most accurate method for identification of coagulase-negative staphylococci (CoNS) (n = 142 strains). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) showed the best results for rapid and accurate CoNS differentiation (99.3% of strains correctly identified). An alternative to this approach could be Vitek2 combined with partial tuf gene sequencing (100% of strains correctly identified when both methods are performed simultaneously).

PubMed Disclaimer

References

    1. Bearman GM, Wenzel RP. 2005. Bacteremias: a leading cause of death. Arch. Med. Res. 36:646–659 - PubMed
    1. Becker K, et al. 2004. Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species. J. Clin. Microbiol. 42:4988–4995 - PMC - PubMed
    1. Bergeron M, et al. 2011. Species identification of staphylococci by amplification and sequencing of the tuf gene compared to the gap gene and by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Eur. J. Clin. Microbiol. Infect. Dis. 30:343–354 - PubMed
    1. Carbonnelle E, et al. 2007. Rapid identification of staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization–time of flight mass spectrometry. J. Clin. Microbiol. 45:2156–2161 - PMC - PubMed
    1. Carpaij N, Willems RJ, Bonten MJ, Fluit AC. 2011. Comparison of the identification of coagulase-negative staphylococci by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and tuf sequencing. Eur. J. Clin. Microbiol. Infect. Dis. 30:1169–1172 - PMC - PubMed

Publication types

LinkOut - more resources