Evaluation of the Yeast Traffic Light PNA FISH probes for identification of Candida species from positive blood cultures
- PMID: 22238445
- PMCID: PMC3318520
- DOI: 10.1128/JCM.06148-11
Evaluation of the Yeast Traffic Light PNA FISH probes for identification of Candida species from positive blood cultures
Abstract
The Yeast Traffic Light PNA FISH kit (YTL) correctly identified Candida spp. in 207/216 (96%) positive blood cultures. Discordant results were seen with known cross-reacting species and cultures containing Candida lambica and Rhodotorula mucilaginosa. The YTL provides rapid, reliable identification of the five common Candida species found in blood cultures.
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References
-
- Alexander BD, Ashley ED, Reller LB, Reed SD. 2006. Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures. Diagn. Microbiol. Infect. Dis. 54:277–282 - PubMed
-
- Enoch DA, Ludlam HA, Brown NM. 2006. Invasive fungal infections: a review of epidemiology and management options. J. Med. Microbiol. 55:809–818 - PubMed
-
- Hall L, et al. 2010. PNA FISH Traffic Light probes for identification of Candida species from blood cultures, abstr C-2068. Abstr. 110th Gen. Meet. Am. Soc. Microbiol., San Diego, CA
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