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. 2012 Aug;31(8):2011-6.
doi: 10.1007/s10096-011-1534-1. Epub 2012 Jan 13.

Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil

C O Beltrame  1 , A M N Botelho, M C Silva-Carvalho, R R Souza, R R Bonelli, M S Ramundo, M A Guimarães, L R Coelho, A M S Figueiredo
Affiliations

Restriction modification (RM) tests associated to additional molecular markers for screening prevalent MRSA clones in Brazil

C O Beltrame et al. Eur J Clin Microbiol Infect Dis. 2012 Aug.

Abstract

In this study, we associated the restriction modification (RM) tests to the polymerase chain reaction (PCR) detection of molecular markers (SCCmec III, seh, agr II-SCCmec IV, and lukSF) for revealing the main methicillin-resistant Staphylococcus aureus (MRSA) clones circulating in Brazil. This simple and rapid approach allowed a precise classification of the MRSA analyzed when compared with pulsed-field gel electrophoresis (PFGE) data.

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