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. 2012 Dec;29(4):2849-57.
doi: 10.1007/s12032-012-0161-8. Epub 2012 Jan 14.

Immunohistochemical detection of CD133 is associated with tumor regression grade after chemoradiotherapy in rectal cancer

Kumiko Hongo  1 , Shinsuke Kazama, Eiji Sunami, Nelson H Tsuno, Koki Takahashi, Hirokazu Nagawa, Joji Kitayama
Affiliations

Immunohistochemical detection of CD133 is associated with tumor regression grade after chemoradiotherapy in rectal cancer

Kumiko Hongo et al. Med Oncol. 2012 Dec.

Abstract

CD133 has been identified as a putative cancer stem cell (CSC) marker in various cancers including colorectal cancer. The relation between CD133 expression and biological characteristics of colorectal cancer remains to be clarified. Protein expression of CD133 was immunohistochemically evaluated in surgical specimens of 225 patients with colorectal cancer who were treated by surgery, as well as those of 78 patients with rectal cancer who received preoperative chemoradiotherapy (CRT) followed by curative resection. The correlation between CD133 expression and clinicopathological features, tumor recurrence and overall survival was analyzed in both populations. Among 225 colorectal cancers without CRT, 93 (41.3%) were positive for CD133 expression, which was enhanced in cases with advanced T stage and venous invasion. Moreover, CD133 was positive in 47 (60.3%) of 78 cases with CRT, which was significantly higher than the CD133-positive rate in non-CRT specimens (P=0.05). Expression of CD133 was independently correlated with the histological tumor regression grade (P<0.01). These results suggest that CD133 is not a distinctive colorectal CSC marker; expression of CD133 is suggested to be one of the key factors associated with resistance to CRT in colorectal cancer.

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Figures

Fig. 1
Fig. 1
Colorectal carcinoma without CRT (a–c) and with CRT (d, e) immunohistochemically stained using CD133 antibody. a Positive immunoreactivity for CD133 (original magnification, ×100). b CD133 expression was seen on the luminal cell surface of colorectal cancer glands, and intraglandular cellular debris was seen in tumor glands with CD133-positive tumor cells (original magnification, ×400). c Tumors showing no staining for CD133. d CD133 expression in residual cancer specimens with CRT (original magnification, ×40). e CD133 immunoreactivity was the same as that in those with surgery alone (original magnification, ×400)
Fig. 2
Fig. 2
Kaplan–Meier plot showing survival time of colorectal cancer patients with surgery alone (a) and of rectal cancer patients with preoperative CRT (b)
See this image and copyright information in PMC

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