doi: 10.1016/0309-1651(79)90125-5.
SV40 DNA injected into Xenopus oocyte nuclei is transcribed by RNA polymerase B
- PMID: 222491
- DOI: 10.1016/0309-1651(79)90125-5
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SV40 DNA injected into Xenopus oocyte nuclei is transcribed by RNA polymerase B
Cell Biol Int Rep.
1979 Mar.
Abstract
SV40 DNA I. injected into Xenopus oocyte nuclei is transcribed. The SV40-specific RNA molecules migrate on sucrose gradients as do viral RNAs formed in infected green monkey cells but a variable proportion of RNA sequences complementary to SV40 DNA is also found in the light region of the gradients. All SV40-specific RNA species seem to be synthesized by RNA polymerase B as their synthesis is completely sensitive to low concentrations (0.1 microgram/ml) of alpha-amanitin. Concomittantly, the formation of SV40-specific proteins (tumor antigens) is inhibited by injecting alpha-amanitin together with the SV40 DNA.
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