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. 2012:842:109-21.
doi: 10.1007/978-1-61779-513-8_6.

Analysis of assembly of secreted mucins

Malin E V Johansson  1 , Gunnar C Hansson
Affiliations

Analysis of assembly of secreted mucins

Malin E V Johansson et al. Methods Mol Biol. 2012.

Abstract

Studies of assembly and secretion of gel-forming mucins are complex. The pulse-chase methods for mucins described here include metabolic radiolabeling and labeling in animals with azido-GalNAc. The labeled mucins are analyzed by composite agarose-polyacrylamide gel electrophoresis and autoradiography or by mucus-preserving tissue fixation and Click-iT(®) chemistry.

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