doi: 10.1016/0014-5793(90)81070-5.
Depurination of plant ribosomes by pokeweed antiviral protein
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- PMID: 2226845
- DOI: 10.1016/0014-5793(90)81070-5
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Depurination of plant ribosomes by pokeweed antiviral protein
FEBS Lett.
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Free article
Abstract
Mammalian ribosomes have been shown to be enzymatically modified by ribosomal inactivating protein (RIPs) via specific depurination of rRNA. Here we report that ribosomes isolated from wheat germ contain intact and undepurinated rRNA and are depurinated by pokeweed antiviral protein (PAP). Pokeweed ribosomes isolated under the same conditions are depurinated. Total RNA isolated from pokeweed in the presence of strong denaturants was found to pbe partially depurinated. We conclude that wheat germ ribosomes are resistant to the endogenous RIP, tritin, but are sensitive to PAP and that pokeweed ribosomes can be depurinated by the N-glycosidase activity of endogenous PAP during isolation.
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