Comparative Study
doi: 10.1016/0014-5793(90)81082-y.
A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence
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- PMID: 2226853
- DOI: 10.1016/0014-5793(90)81082-y
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Comparative Study
A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence
FEBS Lett.
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A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence.FEBS Lett. 1991 Feb 25;279(2):361. doi: 10.1016/0014-5793(91)80187-8. FEBS Lett. 1991. PMID: 2004659 No abstract available.
Abstract
A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in lambda EMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin alpha-subunit has been obtained and sequenced. The principal alpha 1- and alpha 2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 alpha-subunit genes. The nucleotide sequence of the alpha-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.
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