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Comparative Study
. 1990 Oct 29;273(1-2):191-4.
doi: 10.1016/0014-5793(90)81082-y.

A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence

J Jenkins  1 , R G Hiller, J Speirs, J Godovac-Zimmermann
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Free article
Comparative Study

A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence

J Jenkins et al. FEBS Lett. .
Free article

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Abstract

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in lambda EMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin alpha-subunit has been obtained and sequenced. The principal alpha 1- and alpha 2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 alpha-subunit genes. The nucleotide sequence of the alpha-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

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