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. 2012 Feb;27(1):10-8.
doi: 10.1007/s12250-012-3206-4. Epub 2012 Jan 22.

Genetic analysis of the P1 region of human enterovirus 71 strains and expression of the 55 F strainVP1 protein

Jian-qiang Li  1 , Jun-jie Yang, Xiu-juan Fan, Zhen-peng Sun, Yan Sun, Huan Li, Zi-xin Meng, Wei Li
Affiliations

Genetic analysis of the P1 region of human enterovirus 71 strains and expression of the 55 F strainVP1 protein

Jian-qiang Li et al. Virol Sin. 2012 Feb.

Abstract

Enterovirus 71 (EV71) is a member of the Entero-virus genus of the Picornaviridae family and is the major cause of Hand, foot, and mouth disease (HFMD) in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.

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References

    1. Bible J. M., Iturriza-Gomara M., Megson B., et al. Molecular epidemiology of human enterovirus71 in the UnitedKingdomfrom1998 to 2006. J Clin Microbiol. 2006;46:3192–3200. doi: 10.1128/JCM.00628-08. - DOI - PMC - PubMed
    1. Brown B. A., Oberste M. S., Alexander J. P., Jr, et al. Molecular epidemiology and evolution of enterovirus71 strains isolated from1970 to 1998. J Virol. 1999;73(12):9969–9975. - PMC - PubMed
    1. Chan Y. F., Sam I.-C., AbuBaka S. Phylogenetic designation of enterovirus 71 genotype sand subgenotype susing complete genome sequences. Infect Genet Evol. 2010;10:404–412. doi: 10.1016/j.meegid.2009.05.010. - DOI - PubMed
    1. Chen H. L., Huang J. Y., Chu T. W., et al. Expression of VP1 protein in the milk of transgenic mice: A potential oral vaccine protects against enterovirus 71 infection. Vaccine. 2008;26(23):2882–2889. doi: 10.1016/j.vaccine.2008.03.041. - DOI - PubMed
    1. Chumakov M., Voroshilova M., Shindarov L., et al. Enterovirus 71 isolated from cases of epidemic poliomyelitis-like disease in Bulgaria. Arch Virol. 1979;60:329–340. doi: 10.1007/BF01317504. - DOI - PubMed

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