Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Mar;64(2):109-30.
doi: 10.1007/s10616-011-9415-0. Epub 2012 Jan 21.

Flow cytometry: retrospective, fundamentals and recent instrumentation

Julien Picot  1 , Coralie L Guerin, Caroline Le Van Kim, Chantal M Boulanger
Affiliations

Flow cytometry: retrospective, fundamentals and recent instrumentation

Julien Picot et al. Cytotechnology. 2012 Mar.

Abstract

Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Flow cytometry principle
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Arndt-Jovin DJ, Jovin TM. Computer-controlled multiparameter analysis and sorting of cells and particles. J Histochem Cytochem. 1974;22:622–625. doi: 10.1177/22.7.622. - DOI - PubMed
    1. Ashcroft RG, Lopez PA. Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC) J Immunol Methods. 2000;243:13–24. doi: 10.1016/S0022-1759(00)00219-2. - DOI - PubMed
    1. Bonner WA, Hulett HR, Sweet RG, Herzenberg LA. Fluorescence activated cell sorting. Rev Sci Instrum. 1972;43:404–409. doi: 10.1063/1.1685647. - DOI - PubMed
    1. Brecher G, Schneiderman M, Williams GZ. Evaluation of electronic red blood cell counter. Am J Clin Pathol. 1956;26:1439–1449. - PubMed
    1. Chapman GV. Instrumentation for flow cytometry. J Immunol Methods. 2000;243:3–12. doi: 10.1016/S0022-1759(00)00224-6. - DOI - PubMed
The latest specifications of commercial instrumentation can be found on the web pages of the manufacturers:
    1. Amnis Corporation: www.amnis.com
    1. Apogee flow systems: www.apogeeflow.com
    1. Applied Biosystems by Life Technologies: www.appliedbiosystems.com
    1. Bay Bioscience: www.baybio.co.jp
    1. BD Biosciences: www.bdbiosciences.com

LinkOut - more resources