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. 2012 Mar;64(2):109-30.
doi: 10.1007/s10616-011-9415-0. Epub 2012 Jan 21.

Flow cytometry: retrospective, fundamentals and recent instrumentation

Julien Picot  1 , Coralie L Guerin, Caroline Le Van Kim, Chantal M Boulanger
Affiliations

Flow cytometry: retrospective, fundamentals and recent instrumentation

Julien Picot et al. Cytotechnology. 2012 Mar.

Abstract

Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.

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Figures

Fig. 1
Fig. 1
Flow cytometry principle
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The latest specifications of commercial instrumentation can be found on the web pages of the manufacturers:
    1. Amnis Corporation: www.amnis.com
    1. Apogee flow systems: www.apogeeflow.com
    1. Applied Biosystems by Life Technologies: www.appliedbiosystems.com
    1. Bay Bioscience: www.baybio.co.jp
    1. BD Biosciences: www.bdbiosciences.com

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