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. 2011 Jun;24(2):137-42.
doi: 10.1293/tox.24.137. Epub 2011 Jun 30.

Expression of vascular endothelial growth factor (VEGF) associated with histopathological changes in rodent models of osteoarthritis

Fumiko Yamairi  1 , Hiroyuki Utsumi, Yuuichi Ono, Naruyasu Komorita, Masaharu Tanaka, Atsushi Fukunari
Affiliations

Expression of vascular endothelial growth factor (VEGF) associated with histopathological changes in rodent models of osteoarthritis

Fumiko Yamairi et al. J Toxicol Pathol. 2011 Jun.

Abstract

Vascular endothelial growth factor (VEGF) and its receptors have recently reported to be expressed in human osteoarthritis (OA), suggesting that VEGF could be implicated in the pathogenesis of this disease. In the present study, expression of VEGF in the articular cartilage was determined in three different OA models: medial meniscectomy and monoiodoacetate (MIA) injection in rats and age-associated spontaneous joint cartilage destruction in guinea pigs. VEGF was detected by immunohistochemical analysis in the regenerative and hypertrophic chondrocytes, perichondrium and osteophyte areas and chondrocyte clones. Stain intensity of VEGF immunoreactivity increased simultaneously with the degree of cartilage destruction and reparation. These results suggest that VEGF is a key factor in the articular cartilage in human OA and animal OA models.

Keywords: Immunohistochemistry; Medial meniscectomy; Monoiodoacetate (MIA); Osteoarthritis; Vascular endothelial growth factor (VEGF).

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Figures

Fig. 1.
Fig. 1.
Histopathological changes of the articular cartilage of the tibial medial plateau in the frontal plane. The bars indicate 0.5 mm. A, C, E, G: Toluidine blue staining. B, D, F, H: Immunohistochemical staining of VEGF. A, B: Sham operation (Control). C, D: One week after the medial meniscectomy. E, F: Two weeks after the medial meniscectomy. G, H: Five weeks after the medial meniscectomy. The arrows point to VEGF immunoactivity.
Fig. 2.
Fig. 2.
Histopathological changes of the articular cartilage of the femoral condyle after monoiodoacetate (MIA) injection in the frontal plane. The bars indicate 200 μm. A, C: Safranin O staining. B, D: Immunohistochemical staining of VEGF. A, B: Saline injection (Normal). C, D: One week after monoiodoacetate (MIA).
Fig. 3.
Fig. 3.
Histopathological changes of the articular cartilage on age-associated spontaneous OA in guinea pigs. The bars indicate 200 μm. A, C, E: Safranin O staining. B, D, F: Immunohistochemical staining of VEGF. A, B: 10 weeks old. C, D, E, F: 16 months old. A-D: Femoral condyle in the sagittal plane. E, F: Appearance of chondrocyte clones in the cartilage matrix of the tibial medial plateau in the sagittal plane.
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