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. 2011 Dec 7:5:95.
doi: 10.3389/fnsys.2011.00095. eCollection 2011.

The age of enlightenment: evolving opportunities in brain research through optical manipulation of neuronal activity

Jason Jerome  1 , Detlef H Heck
Affiliations

The age of enlightenment: evolving opportunities in brain research through optical manipulation of neuronal activity

Jason Jerome et al. Front Syst Neurosci. .

Abstract

Optical manipulation of neuronal activity has rapidly developed into the most powerful and widely used approach to study mechanisms related to neuronal connectivity over a range of scales. Since the early use of single site uncaging to map network connectivity, rapid technological development of light modulation techniques has added important new options, such as fast scanning photostimulation, massively parallel control of light stimuli, holographic uncaging, and two-photon stimulation techniques. Exciting new developments in optogenetics complement neurotransmitter uncaging techniques by providing cell-type specificity and in vivo usability, providing optical access to the neural substrates of behavior. Here we review the rapid evolution of methods for the optical manipulation of neuronal activity, emphasizing crucial recent developments.

Keywords: caged compounds; digital light processing; light-sensitive ion channels; optical stimulation; optogenetics; spatiotemporal activity; synchrony.

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References

    1. Abbott S. B. G., Stornetta R. L., Fortuna M. G., Depuy S. D., West G. H., Harris T. E., Guyenet P. G. (2009a). Photostimulation of retrotrapezoid nucleus Phox2b-expressing neurons in vivo produces long-lasting activation of breathing in rats. J. Neurosci. 29, 5806.10.1523/JNEUROSCI.1106-09.2009 - DOI - PMC - PubMed
    1. Abbott S. B. G., Stornetta R. L., Socolovsky C. S., West G. H., Guyenet P. G. (2009b). Photostimulation of channelrhodopsin-2 expressing ventrolateral medullary neurons increases sympathetic nerve activity and blood pressure in rats. J. Physiol. (Lond.) 587, 5613–563110.1113/jphysiol.2009.177535 - DOI - PMC - PubMed
    1. Abella I. (1962). Optical double-photon absorption in cesium vapor. Phys. Rev. Lett. 9, 453–45510.1103/PhysRevLett.9.453 - DOI
    1. Adamantidis A. R., Zhang F., Aravanis A. M., Deisseroth K., de Lecea L. (2007). Neural substrates of awakening probed with optogenetic control of hypocretin neurons. Nature 450, 420–42410.1038/nature06310 - DOI - PMC - PubMed
    1. Alilain W. J., Li X., Horn K. P., Dhingra R., Dick T. E., Herlitze S., Silver J. (2008). Light-induced rescue of breathing after spinal cord injury. J. Neurosci. 28, 11862.10.1523/JNEUROSCI.3378-08.2008 - DOI - PMC - PubMed

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