Review: efficiency of physical and chemical treatments on the inactivation of dairy bacteriophages

Abstract

Bacteriophages can cause great economic losses due to fermentation failure in dairy plants. Hence, physical and chemical treatments of raw material and/or equipment are mandatory to maintain phage levels as low as possible. Regarding thermal treatments used to kill pathogenic bacteria or achieve longer shelf-life of dairy products, neither low temperature long time nor high temperature short time pasteurization were able to inactivate most lactic acid bacteria (LAB) phages. Even though most phages did not survive 90°C for 2 min, there were some that resisted 90°C for more than 15 min (conditions suggested by the International Dairy Federation, for complete phage destruction). Among biocides tested, ethanol showed variable effectiveness in phage inactivation, since only phages infecting dairy cocci and Lactobacillus helveticus were reasonably inactivated by this alcohol, whereas isopropanol was in all cases highly ineffective. In turn, peracetic acid has consistently proved to be very fast and efficient to inactivate dairy phages, whereas efficiency of sodium hypochlorite was variable, even among different phages infecting the same LAB species. Both alkaline chloride foam and ethoxylated non-ylphenol with phosphoric acid were remarkably efficient, trait probably related to their highly alkaline or acidic pH values in solution, respectively. Photocatalysis using UV light and TiO(2) has been recently reported as a feasible option to industrially inactivate phages infecting diverse LAB species. Processes involving high pressure were barely used for phage inactivation, but until now most studied phages revealed high resistance to these treatments. To conclude, and given the great phage diversity found on dairies, it is always advisable to combine different anti-phage treatments (biocides, heat, high pressure, photocatalysis), rather than using them separately at extreme conditions.

Keywords: biocides; dairy industry; heat treatments; high pressure; phage infection; photocatalysis.

Figure 1

Thermal destruction kinetics of phages Ib₃ (□), LL-H (■) (Lb. delbruekii), FAGK1 (▲), ATCC 8014-B1 (●) (Lb. plantarum), 021-5 (○), and 0BJ (*) (S. thermophilus), at 72°C using reconstituted skim milk as suspension media. Data adapted from Binetti and Reinheimer (2000), Quiberoni et al. (2003), and Briggiler Marcó et al. (2009).

Figure 2

Viability of dairy bacteriophages after multi-pass high-pressure homogenization treatments at 100 MPa in reconstituted skim milk. Values correspond to viable phage particles (PFU/ml) of (■) untreated samples, and after () one, () three, and (□) five passes. Data adapted from Capra et al. (2009b) and Mercanti et al. (2012).