Production of bovine immunoregulatory molecules by xenogeneic hybrids

Abstract

The study of bovine cytokines and lymphocyte surface markers has been restricted by the lack of stable, long-lived, easily maintained cell lines. To address this problem, bovine mononuclear leucocytes were fused with the mouse thymoma cell line BW5147 to produce interspecies hybrid cell lines and clones. Hybridomas were produced which contained more than 40 chromosomes and expressed one or more bovine leucocyte surface markers, they grew well and were cloned without the use of exogenous factors. Fusion rates were improved when exogenous factors, including recombinant human interleukin-2 (rhIL-2), were added. Some hybrids secreted immunoregulatory factors able to maintain the growth of either an IL-2-dependent bovine T-cell line (C625) and/or preactivated normal bovine B cells. Anti-human IL-2 partially blocked the T-cell growth factor (TCGF) activity produced by these hybrids. The release of growth factors by the hybrid clones 5/AA6/16 and 5/AA6/19 was augmented by concanavalin A stimulation.