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. 2010 Oct;50(4):404-11.
doi: 10.1007/s12088-011-0077-6. Epub 2011 Jan 25.

Characterization of Bradyrhizobium Strains Isolated from Different Varieties of Soybean with 16SrDNA RFLP from Agricultural Land of Madhya Pradesh, India

Anupama Bikrol  1 , Nidhi Saxena, Kiran Singh
Affiliations

Characterization of Bradyrhizobium Strains Isolated from Different Varieties of Soybean with 16SrDNA RFLP from Agricultural Land of Madhya Pradesh, India

Anupama Bikrol et al. Indian J Microbiol. 2010 Oct.

Abstract

Madhya Pradesh is the major soybean contributor in India. The taxonomy of nitrogen fixing bacteria forming symbiotic associations with leguminous plants has been deeply changed in recent years. The use of very sensitive and accurate molecular methods has enabled the detection of large rhizobial diversity. Molecular biotyping and characterization the Bradyrhizobium, isolates from eleven varieties of soybean from agricultural field of Sehore district of Madhya Pradesh is done using 16S rDNA typing. Bradyrhizobia were identified genetically by determining the %Guanine plus Cytosine content of the whole genome, followed by 16S rDNA-RFLP analysis % Guanine plus Cytosine content of all the Bradyrhizobium isolates reflects similarity at generic level among all Bradyrhizobial isolates. Restriction Fragment Length Polymorphism (RFLP) further showed a considerable level of genetic diversity among the Bradyrhizobial isolates. PCR-RFLP of 16S rDNA supported existence of two divergent groups among indigenous Bradyrhizobial isolates, at similarity level of 66, and 75 and 74% of similarity within the group. The technique used was helpful in characterizing Bradyrhizobium isolates to be used as inoculants for improving productivity of agricultural land of Madhya Pradesh (India).

Keywords: % Guanine plus Cytosine content (% G + C); 16S rDNA; Bradyrhizobium sp; DNA fingerprinting; Genetic diversity; Soybean.

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Figures

Fig. 1
Fig. 1
Restriction enzyme TaqI treated 16S rDNA segments of isolated strains of Bradyhizobium sp. on 2% agarose gel
Fig. 2
Fig. 2
Dendogram based on RFLP of 16S rDNA segment of Bradyrhizobium isolates using TaqI restriction enzyme
Fig. 3
Fig. 3
Restriction enzyme MboI treated 16S rDNA segments of isolated strains of Bradyrhizobium sp. on 2% agarose gel
Fig. 4
Fig. 4
Dendogram based on RFLP of 16S rDNA segment of Bradyrhizobium isolates using MboI restriction enzyme
Fig. 5
Fig. 5
Dendogram based on analysis of 16S rDNA segment of Bradyrhizobium
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