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. 2011:2011:768637.
doi: 10.5402/2011/768637. Epub 2011 Nov 16.

Short-Term Calorie Restriction in Early Life Attenuates the Development of Proteinuria but Not Glucose Intolerance in Type 2 Diabetic OLETF Rats

Daisuke Nakano  1 , Suwarni Diah, Kento Kitada, Hirofumi Hitomi, Hirohito Mori, Tsutomu Masaki, Hiroyuki Kobori, Akira Nishiyama
Affiliations

Short-Term Calorie Restriction in Early Life Attenuates the Development of Proteinuria but Not Glucose Intolerance in Type 2 Diabetic OLETF Rats

Daisuke Nakano et al. ISRN Endocrinol. 2011.

Abstract

Childhood obesity is becoming more prevalent; however, the influence of obesity or dieting during childhood on outcomes in adulthood is poorly understood. The aim of this study was to examine the effects of short-term calorie restriction (CR) and high-calorie feeding with high-fat or high-sucrose diets during early life on the development of glucose tolerance and diabetic nephropathy in later life of Otsuka Long-Evans Tokushima fatty (OLETF) rats. Neither high-calorie intake nor CR at 7-13 weeks of age affected glucose tolerance of 27-week-old OLETF rats. On the other hand, proteinuria was lower at 27 weeks of age in CR rats than in the other rats. These results suggest that short-term CR at a young age protects against the development of renal injury in later life. In contrast, short-term high-calorie intake or CR at a young age does not appear to affect glucose metabolism in later life.

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Figures

Figure 1
Figure 1
Changes in plasma glucose levels during oral glucose tolerance tests performed at 13 (a) and 27 (b) weeks of age, and the calculated total areas under the glucose curves at 13 (c) and at 27 (d) weeks of age. Compared with the control LETO rats, the normal diet-fed, high-sucrose-fed, and high-fat-fed OLETF rats showed higher glucose levels following the oral glucose load at 13 weeks of age. The changes in blood glucose levels following the oral glucose load in calorie-restricted OLETF rats were almost identical to those in LETO rats at 13 weeks of age. Blood glucose levels were significantly greater in all OLETF rats than in LETO rats, and there were no differences in blood glucose level between any of the OLETF rats at 27 weeks of age. L: LETO + normal diet; O: OLETF + normal diet; HS: OLETF + high-sucrose diet; HF: OLETF + high-fat diet; CR: OLETF + calorie restriction. *P < 0.05 versus LETO rats, # P < 0.05 versus normal diet-fed OLETF rats.
Figure 2
Figure 2
Urinary protein excretion levels (a) and renal morphological changes (b). Proteinuria was greater in all OLETF rats than in LETO rats and was greater in high-fat-fed rats than in normal-diet-fed rats at 13 weeks of age. Proteinuria was further increased at 27 weeks of age in all OLETF rats, although the increment in proteinuria in calorie-restricted OLETF rats was significantly less than that in normal-diet-fed OLETF rats. As shown in (b), the PAS-positive area in the kidney was similar among the 27-week-old rats. L: LETO + normal diet; O: OLETF + normal diet; HS: OLETF + high-sucrose diet; HF: OLETF + high-fat diet; CR: OLETF + calorie restriction. *P < 0.05 versus LETO rats, # P < 0.05 versus normal-diet-fed OLETF rats.
Figure 3
Figure 3
mRNA expression of SIRT1 (a and b) and 3 (c and d) in the kidneys of 13-week-old (a and c) and 27-week-old rats (b and d). There were no differences in either SIRT1 or 3 expression in the kidneys between any of the groups at either time. L: LETO + normal diet; O: OLETF + normal diet; HS: OLETF + high-sucrose diet; HF: OLETF + high-fat dietl CR: OLETF + calorie restriction.
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