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. 2012 Mar 5;51(10):2405-8.
doi: 10.1002/anie.201107797. Epub 2012 Jan 27.

Temperature-responsive "catch and release" of proteins by using multifunctional polymer-based nanoparticles

Keiichi Yoshimatsu  1 , Benjamin K Lesel, Yusuke Yonamine, John M Beierle, Yu Hoshino, Kenneth J Shea
Affiliations

Temperature-responsive "catch and release" of proteins by using multifunctional polymer-based nanoparticles

Keiichi Yoshimatsu et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
(a) Preparation of multifunctional polymer NPs. APS: ammonium persulfate. SDS: sodium dodecyl sulfate (b) Schematic illustration of temperature-responsive “catch-and-release” of a protein by multifunctional polymer NPs. LCST: lower critical solution temperature.
Figure 2
Figure 2
(a) Depletion of lysozyme (5 μg/mL) from solutions after incubation with NPs (800 μg/mL) at room temp. (b) Binding selectivity of NP2 at room temp. NP2: 2.0 mg/mL. Protein conc: 200 μg/mL. (c) Temperature-responsive “catch-and-release” property of lysozyme (5 μg/mL) by NP2 (2.0 mg/experiments are carried out in PBS (35 mM sodium-phosphate buffer containing 150 mM NaCl, pH 7.3)
Figure 3
Figure 3
(a) Schematic of “catch-and-release” lysozyme purification from egg whites. (b) SDS-PAGE analysis of flow-through fraction (FT) and cold elution fraction (CE) after Coomassie Brilliant Blue R-250 staining. Lane 1 and 6: molecular weight markers. Lane 2: FT from 1st cycle. Lane 3: CE from 1st cycle. Lane 4: FT from 2nd cycle. Lane 5: CE fraction from 2nd cycle. A control experiment was carried out in absence of NPs. Lane 7: FT from the control. Lane 8: CE from the control.
See this image and copyright information in PMC

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