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Comparative Study
. 1990 Nov;172(11):6308-15.
doi: 10.1128/jb.172.11.6308-6315.1990.

Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase

A M Cleton-Jansen  1 , N Goosen, O Fayet, P van de Putte
Affiliations
Comparative Study

Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase

A M Cleton-Jansen et al. J Bacteriol. 1990 Nov.

Abstract

Escherichia coli contains pyrroloquinoline quinone-dependent glucose dehydrogenase. We cloned and sequenced the gene (gcd) encoding this enzyme and showed that the derived amino acid sequence is highly homologous to that of the gdhA gene product of Acinetobacter calcoaceticus. Stretches of homology also exist between the amino acid sequence of E. coli glucose dehydrogenase and other pyrroloquinoline quinone-dependent dehydrogenases from several bacterial species. The position of gcd on the chromosomal map of E. coli was determined to be at 3.1 min.

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References

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