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. 2012 Jan 30;9(2):117-8.
doi: 10.1038/nmeth.1865.

Improved Mos1-mediated transgenesis in C. elegans

Christian Frøkjær-Jensen, M Wayne Davis, Michael Ailion, Erik M Jorgensen

Improved Mos1-mediated transgenesis in C. elegans

Christian Frøkjær-Jensen et al. Nat Methods. .
No abstract available

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Figures

Figure 1
Figure 1
Improvements to Mos1-based genome manipulation. (a) A plasmid expressing transposase under the indicated promoters was coinjected with low DNA concentration (32.5 ng/ul) of a 4.4 kb transgene. The plot shows insertion frequency into the ttTi5605 locus. (b) The plot shows the frequency of a 5 kb targeted deletion of dpy-13. Pglh-2 data from Frøkjær-Jensen et al (2010)) using the indicated selection markers (see Supplementary Methods for discussion). (c) Insertion frequency with higher total DNA concentrations (~100 ng/ul) and in the presence of the negative selection marker peel-1. Error bars, 95 % confidence intervals, significance was determined with Fischer’s exact test
See this image and copyright information in PMC

References

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