Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Sep;28(9):1968-73.
doi: 10.1128/jcm.28.9.1968-1973.1990.

Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay

L Bobo  1 , F Coutlee, R H Yolken, T Quinn, R P Viscidi
Affiliations

Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay

L Bobo et al. J Clin Microbiol. 1990 Sep.

Erratum in

Abstract

A sensitive and specific system for detection of amplified Chlamydia trachomatis DNA from cervical specimens by fluorometric quantitation in an enzyme immunoassay (EIA) format (polymerase chain reaction [PCR]-EIA) is described. The primers selected for PCR-amplified DNA were from the 15 serovars of C. trachomatis and two strains of Chlamydia pneumoniae (TWAR). One strain of Chlamydia psittaci (Borg) was not amplified. One hundred four previously cultured cervical specimens were evaluated. Forty-six culture-positive specimens containing from 1+ to 4+ inclusion bodies were all positive by PCR-EIA. Of 58 culture-negative specimens, 2 were repeatedly positive and were nonreactive with control probes. This assay system represents a sensitive and specific combination of technologies for the quantitative detection of C. trachomatis DNA directly from a body fluid.

PubMed Disclaimer

References

    1. Science. 1985 Dec 20;230(4732):1350-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6977-81 - PubMed
    1. J Clin Microbiol. 1987 Oct;25(10):1911-6 - PubMed
    1. J Virol. 1989 Jan;63(1):64-75 - PubMed
    1. J Med Virol. 1989 Feb;27(2):105-11 - PubMed

Publication types

MeSH terms