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. 1990 Sep;28(9):2094-8.
doi: 10.1128/jcm.28.9.2094-2098.1990.

High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis

W R Butler  1 , J O Kilburn
Affiliations

High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis

W R Butler et al. J Clin Microbiol. 1990 Sep.

Abstract

Rapidly growing mycobacteria of clinical significance were identified by mycolic acids detected with high-performance liquid chromatography. Mycolic acids from whole cells were extracted, derivatized, and detected by a modified high-performance liquid chromatography procedure in less than 3 h. Use of an internal standard allowed differentiation of Mycobacterium chelonae and Mycobacterium fortuitum by comparison of relative retention times. Peak height ratios were used for subidentification of M. chelonae strains; however, M. fortuitum and Mycobacterium smegmatis could not be separated by this system.

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References

    1. J Biochem. 1980 Jul;88(1):211-21 - PubMed
    1. J Clin Microbiol. 1979 Oct;10(4):506-14 - PubMed
    1. Tubercle. 1981 Dec;62(4):285-7 - PubMed
    1. J Bacteriol. 1982 Aug;151(2):828-37 - PubMed
    1. J Clin Microbiol. 1982 Aug;16(2):400-2 - PubMed

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