Enzyme immunoassay for pigeon breeders' disease without the use of extraneous antibody

Abstract

A rapid enzyme immunoassay technique is described for the detection in human sera of antibodies of any class to pigeon antigens. The method is based on binding human antibodies in excess to polystyrene-fixed antigens from pigeon droppings or pigeon serum, followed by capture of the same antigens coupled to peroxidase. A very good correlation was found of titers obtained by means of this 60-min assay with traditional sandwich immunoassay and with precipitation- and complement - fixation tests.