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. 2012 Apr;61(4):581-5.
doi: 10.1007/s00262-012-1205-9.

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

Ravindra A De Silva  1 , Dananjaya K Appulage, Halina Pietraszkiewicz, Kevin R Bobbitt, Joe Media, JiaJiu Shaw, Fred A Valeriote, Peter R Andreana
Affiliations

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

Ravindra A De Silva et al. Cancer Immunol Immunother. 2012 Apr.

Abstract

The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; a-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
a Flow cytometry results using human tumor cell lines expressing the Tn hapten (2). Anti-sera (diluted at 1:250) from Tn-PS A1 (3)-primed C57BL/6 mice (red trace) and anti-sera (diluted at 1:250) from PS A1 (1)-primed C57BL/6 mice (blue trace) along with staining agent 2° IgG3 Ab conjugated with Alexa Fluor® 488 dye (green trace). FACS analysis shows Ab binding to MDA-231, MCF-7, Jurkat, JurkatTAg, and Panc-1 but not glioblastoma U251 (see discussion). PBMCs and bone marrow cells were used as controls. Results are representative of two replicate experiments. b Percentage of cells staining positive treated with sera from Tn-PS A1-inoculated animals compared with the percentage of cells staining with sera from PS A1 vaccinated animals. Percent of positive stained cells incubated with Tn-PS A1 sera were divided by those obtained from cells incubated with PS A1. Results represent the fold change from the average of two replicate experiments
Scheme 1
Scheme 1
The Tn-PS A1 construct (3) from the glycoconjugation of PS A1 (1) with Tn hapten (2)
See this image and copyright information in PMC

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