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Comparative Study
. 2012 Apr;50(4):1415-8.
doi: 10.1128/JCM.06326-11. Epub 2012 Feb 1.

Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ingrid Wybo  1 , Oriane Soetens, Annelies De Bel, Fedoua Echahidi, Ellen Vancutsem, Kristof Vandoorslaer, Denis Piérard
Affiliations
Comparative Study

Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ingrid Wybo et al. J Clin Microbiol. 2012 Apr.

Abstract

The performance of matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) for species identification of Prevotella was evaluated and compared with 16S rRNA gene sequencing. Using a Bruker database, 62.7% of the 102 clinical isolates were identified to the species level and 73.5% to the genus level. Extension of the commercial database improved these figures to, respectively, 83.3% and 89.2%. MALDI-TOF MS identification of Prevotella is reliable but needs a more extensive database.

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References

    1. Berger P, Adekambi T, Mallet MN, Drancourt M. 2005. Prevotella massiliensis sp. nov. isolated from human blood. Res. Microbiol. 156:967–973 - PubMed
    1. Bizzini A, Greub G. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16:1614–1619 - PubMed
    1. Citron DM, Poxton IR, Baron EJ. 2007. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic Gram-negative rods, p 911–932 In Murray PE, Baron EJ, Pfaller MA, Jorgensen JH, Landry ML. (ed), Manual of clinical microbiology, 9th ed ASM Press, Washington, DC
    1. Clinical and Laboratory Standards Institute 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved guideline. CLSI document MM18-A Clinical and Laboratory Standards Institute, Wayne, PA
    1. Coenye T, Goris J, Spilker T, Vandamme P, LiPuma JJ. 2002. Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J. Clin. Microbiol. 40:2062–2069 - PMC - PubMed

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