Fine Mapping of Loci on BTA2 and BTA26 Associated with Bovine Viral Diarrhea Persistent Infection and Linked with Bovine Respiratory Disease in Cattle

Abstract

Bovine respiratory disease (BRD) is considered to be the most costly infectious disease in the cattle industry. Bovine viral diarrhea virus (BVDV) is one of the pathogens involved with the BRD complex of disease. BVDV infection also negatively impacts cow reproduction and calf performance. Loci associated with persistently infected animals (BVD-PI) and linked with BRD have previously been identified near 14 Mb on bovine chromosome 2 (BTA2) and 15.3 Mb on bovine chromosome 26 (BTA26). The objective of this study was to refine the loci associated with BVD-PI and linked with BRD. Association testing for BVD-PI was performed on a population of 65 BVD-PI calves, 51 of their dams, and 60 unaffected calves (controls) with 142 single nucleotide polymorphisms (SNPs) on BTA2 and 173 SNPs on BTA26. Comparisons were made between BVD-PI calves and controls calves and the dams of BVD-PI calves and controls calves. For the linkage analysis of BRD, the same markers were used to genotype two half-sib families consisting of the sires and 72 BRD positive and 148 BRD negative offspring. Using an allelic chi-square test, 11 loci on BTA2 and 8 loci on BTA26 were associated with the dams of the BVD-PI calves (P < 0.05) and 4 loci on BTA2 and 11 loci on BTA26 were associated with BVD-PI calves. This demonstrates that although some of the loci on BTA2 and BTA26 are jointly involved in the fetal and dam response to BVD-PI infection, there are loci that are solely associated with the maternal or fetal susceptibility to disease. One locus on BTA2 and two loci on BTA26 were found to be linked (P < 0.05) with BRD. The regions linked with BRD were also associated with BVD-PI demonstrating that both the broad (BRD) and narrow (BVD-PI) definition of disease identified shared genomic regions as important in disease susceptibility. These results further refined the loci associated with BVD-PI and linked with BRD.

Keywords: association linkage; bovine respiratory disease; bovine viral diarrhea; genetics; single nucleotide polymorphism.

Figure 1

Linkage of bovine respiratory disease with BTA2 with a single locus model in two half-sib sire families. The computed F-statistic is shown on the y-axis and chromosomal location is given in centimorgans (cM) on the x-axis. The boxed regions identify the locus linked with bovine respiratory disease (P < 0.05) after 5,000 permutations. The horizontal line represents the significance threshold of P = 0.05.

Figure 2

Linkage of bovine respiratory disease with BTA26 with a two locus model in two half-sib sire families. The computed F-statistic is shown on the y-axis and the relative distance between each SNP is given in centimorgans (cM) on the x-axis. The boxed regions identify the two loci linked with bovine respiratory disease (P < 0.05) after 5,000 permutations. The horizontal line represents the significance threshold of P = 0.05.