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. 2012 Apr;5(4):1049-52.
doi: 10.3892/mmr.2012.778. Epub 2012 Feb 3.

TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons

Jun Zhou  1 , Shang-Long Yao, Cheng-Xiang Yang, Ji-Ying Zhong, Han-Bing Wang, Yan Zhang
Affiliations

TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons

Jun Zhou et al. Mol Med Rep. 2012 Apr.

Abstract

The present study was conducted to determine whether the activation of TRESK in the dorsal root ganglion (DRG) by the TRESK gene recombinant adenovirus vector inhibits the capsaicin-evoked substance P (SP) release using a radioimmunoassay. TRESK is an outwardly rectifying K+ current channel that contributes to the resting potential and is the most important background potassium channel in DRG. Previous studies have shown that neuropathic pain (NP) is closely related to the regulation of certain potassium channels in DRG neurons, while DRG-released SP is important in the peripheral mechanism of NP. In the present study, the TRESK gene adenovirus vector significantly enhanced the TRESK mRNA and protein of the cultured rat DRG neurons. Radioimmunoassay analysis revealed that the capsaicin‑mediated SP release was significantly inhibited by the TRESK gene recombinant adenovirus vector in rat DRG neurons. These findings suggest that TRESK plays a role in adjusting the release of SP in DRG, which is related to NP.

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Figures

Figure 1
Figure 1
Comparison of TRESK mRNA, TRESK protein and capsaicin-induced SP release from the cultured DRG neurons of each group. (A) TRESK mRNA was detected by RT-PCR. (B) TRESK proteins were detected by western blotting. (C) Capsaicin-induced SP release from DRG neurons was assessed by RNA. Values are expressed as the means ± SD of five independent experiments. *P<0.05; **P<0.01.
Figure 1
Figure 1
Comparison of TRESK mRNA, TRESK protein and capsaicin-induced SP release from the cultured DRG neurons of each group. (A) TRESK mRNA was detected by RT-PCR. (B) TRESK proteins were detected by western blotting. (C) Capsaicin-induced SP release from DRG neurons was assessed by RNA. Values are expressed as the means ± SD of five independent experiments. *P<0.05; **P<0.01.
Figure 1
Figure 1
Comparison of TRESK mRNA, TRESK protein and capsaicin-induced SP release from the cultured DRG neurons of each group. (A) TRESK mRNA was detected by RT-PCR. (B) TRESK proteins were detected by western blotting. (C) Capsaicin-induced SP release from DRG neurons was assessed by RNA. Values are expressed as the means ± SD of five independent experiments. *P<0.05; **P<0.01.
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References

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