This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!

Skip to main page content

Add to Collections

Your saved search

Name of saved search:

Search terms:

Test search terms

Would you like email updates of new search results?

Yes
No

Email: (change)

Frequency:

Which day?

Which day?

Report format:

Send at most:

Send even when there aren't any new results

Optional text in email:

Your RSS Feed

Comment

. 2012 Jan 17;109(3):649-50.

doi: 10.1073/pnas.1119350109. Epub 2012 Jan 17.

Gaining confidence in high-throughput screening

Jérôme Bibette¹

Affiliations

PMID: 22308304
PMCID: PMC3271920
DOI: 10.1073/pnas.1119350109

Comment

Gaining confidence in high-throughput screening

Jérôme Bibette. Proc Natl Acad Sci U S A. 2012.

. 2012 Jan 17;109(3):649-50.

doi: 10.1073/pnas.1119350109. Epub 2012 Jan 17.

Author

Jérôme Bibette¹

Affiliation

¹ Laboratoire Colloïdes et Matériaux Divisés, UPMC/CNRS UMR 7195, ESPCI ParisTech, 75231 Paris, France. jerome.bibette@espci.fr

PMID: 22308304
PMCID: PMC3271920
DOI: 10.1073/pnas.1119350109

No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1. — Fig. 1.
Comparison of (A) a conventional dose–response curve produced using microplates (10 replicates at eight concentrations) and (B) a high-resolution dose–response curves produced using droplet-based microfluidics (data from 11,113 droplets). The curves are for inhibition of the enzyme β-galactosidase by 2-phenylethyl β-d-thiogalactoside. The 95% confidence limits for the fitted IC₅₀ and Hill slope values (Inset in A and B) are approximately an order of magnitude more precise for the high-resolution dose–response curve (5).

See this image and copyright information in PMC

Comment on

High-resolution dose-response screening using droplet-based microfluidics.
Miller OJ, El Harrak A, Mangeat T, Baret JC, Frenz L, El Debs B, Mayot E, Samuels ML, Rooney EK, Dieu P, Galvan M, Link DR, Griffiths AD. Miller OJ, et al. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):378-83. doi: 10.1073/pnas.1113324109. Epub 2011 Dec 27. Proc Natl Acad Sci U S A. 2012. PMID: 22203966 Free PMC article.

Similar articles

High-resolution dose-response screening using droplet-based microfluidics.
Miller OJ, El Harrak A, Mangeat T, Baret JC, Frenz L, El Debs B, Mayot E, Samuels ML, Rooney EK, Dieu P, Galvan M, Link DR, Griffiths AD. Miller OJ, et al. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):378-83. doi: 10.1073/pnas.1113324109. Epub 2011 Dec 27. Proc Natl Acad Sci U S A. 2012. PMID: 22203966 Free PMC article.
Micro- and nanofluidic systems for high-throughput biological screening.
Hong J, Edel JB, deMello AJ. Hong J, et al. Drug Discov Today. 2009 Feb;14(3-4):134-46. doi: 10.1016/j.drudis.2008.10.001. Epub 2008 Dec 4. Drug Discov Today. 2009. PMID: 18983933 Review.
Microfluidics: Analog-to-digital drug screening.
Wootton RC, Demello AJ. Wootton RC, et al. Nature. 2012 Feb 29;483(7387):43-4. doi: 10.1038/483043a. Nature. 2012. PMID: 22382977 No abstract available.
BIOCHEMISTRY. DNA helps build molecular libraries for drug testing.
Gura T. Gura T. Science. 2015 Dec 4;350(6265):1139-40. doi: 10.1126/science.350.6265.1139. Science. 2015. PMID: 26785450 No abstract available.
Repurposing libraries of eukaryotic protein kinase inhibitors for antibiotic discovery.
Walsh CT, Fischbach MA. Walsh CT, et al. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1689-90. doi: 10.1073/pnas.0813405106. Epub 2009 Feb 4. Proc Natl Acad Sci U S A. 2009. PMID: 19193851 Free PMC article. Review. No abstract available.

See all similar articles

Cited by

Creative use of analytical techniques and high-throughput technology to facilitate safety assessment of engineered nanomaterials.
Liu Q, Wang X, Xia T. Liu Q, et al. Anal Bioanal Chem. 2018 Sep;410(24):6097-6111. doi: 10.1007/s00216-018-1289-y. Epub 2018 Aug 1. Anal Bioanal Chem. 2018. PMID: 30066194 Free PMC article. Review.
Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles.
Kusi-Appiah AE, Mastronardi ML, Qian C, Chen KK, Ghazanfari L, Prommapan P, Kübel C, Ozin GA, Lenhert S. Kusi-Appiah AE, et al. Sci Rep. 2017 Mar 8;7:43731. doi: 10.1038/srep43731. Sci Rep. 2017. PMID: 28272505 Free PMC article.
Oxidative Reactivities of 2-Furylquinolines: Ubiquitous Scaffolds in Common High-Throughput Screening Libraries.
Olson ME, Abate-Pella D, Perkins AL, Li M, Carpenter MA, Rathore A, Harris RS, Harki DA. Olson ME, et al. J Med Chem. 2015 Sep 24;58(18):7419-30. doi: 10.1021/acs.jmedchem.5b00930. Epub 2015 Sep 11. J Med Chem. 2015. PMID: 26358009 Free PMC article.
Pharmacological Inhibition of LSD1 for Cancer Treatment.
Yang GJ, Lei PM, Wong SY, Ma DL, Leung CH. Yang GJ, et al. Molecules. 2018 Dec 4;23(12):3194. doi: 10.3390/molecules23123194. Molecules. 2018. PMID: 30518104 Free PMC article. Review.
Mechanisms, Challenges, and Opportunities of Dual Ni/Photoredox-Catalyzed C(sp²)-C(sp³) Cross-Couplings.
Yuan M, Gutierrez O. Yuan M, et al. Wiley Interdiscip Rev Comput Mol Sci. 2022 May-Jun;12(3):e1573. doi: 10.1002/wcms.1573. Epub 2021 Sep 21. Wiley Interdiscip Rev Comput Mol Sci. 2022. PMID: 35664524 Free PMC article.

See all "Cited by" articles

References

1. Dove A. Drug screening—beyond the bottleneck. Nat Biotechnol. 1999;17:859–863. - PubMed
1. Mayr LM, Bojanic D. Novel trends in high-throughput screening. Curr Opin Pharmacol. 2009;9:580–588. - PubMed
1. Malo N, Hanley JA, Cerquozzi S, Pelletier J, Nadon R. Statistical practice in high-throughput screening data analysis. Nat Biotechnol. 2006;24:167–175. - PubMed
1. Lipinski C, Hopkins A. Navigating chemical space for biology and medicine. Nature. 2004;432:855–861. - PubMed
1. Miller OJ, et al. High-resolution dose–response screening using droplet-based microfluidics. Proc Natl Acad Sci USA. 2011;109:378–383. - PMC - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions

Substances

Actions

LinkOut - more resources

Full Text Sources