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. 2012 Feb 6:5:87.
doi: 10.1186/1756-0500-5-87.

The PCNA pseudogenes in the human genome

Ivaylo Stoimenov  1 , Anne Lagerqvist
Affiliations

The PCNA pseudogenes in the human genome

Ivaylo Stoimenov et al. BMC Res Notes. .

Abstract

Background: The proliferating cell nuclear antigen (PCNA) is a key protein in the eukaryotic DNA replication and cell proliferation. Following the cloning and characterisation of the human PCNA gene, the question of the existence of pseudogenes in the human genome was raised.

Findings: In this short communication we summarise the existing information about the PCNA pseudogenes and critically assess their status.

Conclusions: We propose the existence of at least four valid PCNA pseudogenes, PCNAP1, PCNAP2, LOC392454 and LOC390102. We would like to recommend assignment of a name for LOC392454 as "proliferating cell nuclear antigen pseudogene 3" (alias PCNAP3) and a name for LOC390102 as "proliferating cell nuclear antigen pseudogene 4" (alias PCNAP4). We prompt for more critical evaluation of the existence of a PCNA pseudogene, designated as PCNAP.

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Figures

Figure 1
Figure 1
Alignment of the sequences of the PCNA pseudogene published by Ku et al. [6]and LOC392454, including 5'- and 3'-flanking regions of LOC392454.
Figure 2
Figure 2
Predicted EcoRI restriction pattern of the two loci on human chromosome X, containing a proposed PCNA pseudogene. The restriction endonuclease sites of AvaI are denoted for additional clarity. A) PCNAP with additional 8 kb region (4 kb flanking in 5'- and 4 kb flanking in 3'-direction); B) LOC392454 with additional 8 kb region (4 kb flanking in 5'- and 4 kb flanking in 3'-direction). EcoRI (restriction endonuclease I from Escherichia coli), AvaI (restriction endonuclease I from Anabaena variabilis).
Figure 3
Figure 3
Alignment of the sequences of the PCNA gene and the proposed PCNA pseudogenes (PCNAP1, PCNAP2, LOC392454, LOC390102 and PCNAP).
See this image and copyright information in PMC

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