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. 2012;76(2):384-7.
doi: 10.1271/bbb.110673. Epub 2012 Feb 7.

Streptomyces nodosus host strains optimized for polyene glycosylation engineering

Niamh Stephens  1 , Bernard Rawlings, Patrick Caffrey
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Free article

Streptomyces nodosus host strains optimized for polyene glycosylation engineering

Niamh Stephens et al. Biosci Biotechnol Biochem. 2012.
Free article

Abstract

The AmphDI glycosyltransferase transfers a mycosaminyl sugar residue from GDP onto 8-deoxyamphoteronolide B, the aglycone of the antifungal amphotericin B. In this study the amphDI gene was inactivated in Streptomyces nodosus strains lacking the AmphN cytochrome P450. The new mutants produced 8-deoxy-16-methyl-16-descarboxyl amphoteronolides in high yield. These strains and aglycones should prove valuable for in vivo and in vitro glycosylation engineering.

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