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. 2012 Feb;14(2):138-46.
doi: 10.1177/1098612X11432236.

Analytical performance of commercially-available assays for feline insulin-like growth factor 1 (IGF-1), adiponectin and ghrelin measurements

Asta Tvarijonaviciute  1 , Alexander J German, Silvia Martínez-Subiela, Fernando Tecles, Jose J Ceron
Affiliations

Analytical performance of commercially-available assays for feline insulin-like growth factor 1 (IGF-1), adiponectin and ghrelin measurements

Asta Tvarijonaviciute et al. J Feline Med Surg. 2012 Feb.

Abstract

The objective of this study was to evaluate three commercially-available human assays for the determination of adiponectin, ghrelin and insulin-like growth factor 1 (IGF-1) concentrations in feline serum samples. Intra- and interassay coefficients of variation were lower than 20%, 15% and 6% for adiponectin, ghrelin and IGF-1 assays, respectively. Dilutions of feline serum pools resulted in linear regression equations in all kits. Mean recovery of adiponectin, ghrelin and IGF-1 assays were 107%, 102% and 105%, respectively. Significant differences were detected in adiponectin and ghrelin concentrations between lean and obese cats (P <0.05 in both cases), but there was no difference in IGF-1 concentrations (P = 0.12).

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Conflict of interest statement

The authors declare that there is no conflict of interest.

Figures

Figure 1
Figure 1
Linearity under dilution of two feline serum pools with different adiponectin (A), ghrelin (B) and IGF-1 (C) concentrations
Figure 2
Figure 2
Interferograms corresponding to the effect of triglycerides (A), haemoglobin (B) and bilirubin (C) concentrations on adiponectin determination in two feline serum pools. In these graphs, results obtained with the two pools are presented with open boxes and with open triangles, respectively. X-axes show increasing concentrations of haemoglobin, triglycerides or bilirubin, whilst Y-axes show percentage of change in adiponectin [(Vƒ/V0)×100]. Vƒ = final value; V0 = original value
Figure 3
Figure 3
Interferograms corresponding to the effect of triglycerides (A), haemoglobin (B) and bilirubin (C) concentrations on ghrelin determination in two feline serum pools. In these graphs, results obtained with the two pools are presented with open boxes and with open triangles, respectively. X-axes show increasing concentrations of haemoglobin, triglycerides or bilirubin, whilst Y-axes show percentage of change in ghrelin [(Vƒ/ V0)×100]. V = final value; V0 = original value
Figure 4
Figure 4
Interferograms corresponding to the effect of triglycerides (A), haemoglobin (B), and bilirubin (C) concentrations on IGF-1 determination in two feline serum pools. In these graphs, results obtained with the two pools are presented with open boxes and with open triangles, respectively. X-axes show increasing concentrations of haemoglobin, triglycerides, or bilirubin, whilst Y-axes show percentage of change in IGF-1 [(Vƒ/ V0)×100]. Vƒ = final value; V0 = original value
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