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Comparative Study
. 2012 Feb;14(2):165-8.
doi: 10.1177/1098612X11429224.

In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells

Tracy L Webb  1 , Jessica M Quimby, Steven W Dow
Affiliations
Comparative Study

In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells

Tracy L Webb et al. J Feline Med Surg. 2012 Feb.

Abstract

Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for a variety of different diseases in human and veterinary medicine. At present, MSC are most often collected from bone marrow (BM) or adipose tissue (AT) and enriched and expanded in vitro before being transferred into recipients. However, little is known regarding the culture characteristics of feline BM-derived (BM-MSC) versus AT-derived MSC (AT-MSC). We compared BM-MSC and AT-MSC from healthy cats with respect to in vitro growth and cell surface phenotype. Mesenchymal stem cells isolated from AT proliferated significantly faster than BM-MSC. Phenotypic differences between BM-MSC and AT-MSC were not present in the surface markers assessed. We conclude that BM-MSC and AT-MSC are similar phenotypically but that cultures of AT-MSC are easier to generate because of their higher intrinsic proliferative rate. Thus, AT-MSC may be the preferred MSC for clinical applications where rapid and efficient generation of MSC is important.

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Conflict of interest statement

The authors declare that there is no conflict of interest.

Figures

Figure 1
Figure 1
Feline adipose tissue-derived MSCs (AT-MSCs) proliferate more rapidly than bone marrow-derived MSCs (BM-MSCs) from the same animals after 72 and 120 h in culture. Proliferation of AT-MSCs and BM-MSCs was measured by standard MTT assay in triplicate at baseline (1 h), 24, 72, and 120 h (*= P <0.01). Results are representative of two separate experiments. Culture time (hours) is shown on the x axis, and average absorbance value is shown on the y axis
Figure 2
Figure 2
Feline adipose-derived MSCs (AT-MSCs) demonstrate similar surface marker expression as feline bone marrow-derived MSCs (BM-MSCs). Primary cultures of feline MSCs were passaged 2–5 times, collected by trypsinization, and immunostained for assessment of CD4, MHC class II, CD44, CD105, and CD90 percent expression by flow cytometry. Data is representative of two separate experiments (n = 4). The specific surface marker examined is listed on the x axis, and the average percent expression is shown on the y axis
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