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. 1979 Jun;76(6):2679-83.
doi: 10.1073/pnas.76.6.2679.

Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA

A Dasgupta, M H Baron, D Baltimore

Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA

A Dasgupta et al. Proc Natl Acad Sci U S A. 1979 Jun.

Abstract

The soluble phase of the cytoplasm of poliovirus-infected cells contains an enzymatic activity able to copy RNA without an added primer. This replicase activity has been purified 60-fold; it is absent from uninfected cells. Poly(U) polymerase activity copurifies with replicase activity. Although less pure replicase fractions copy a variety of RNAs, purer fractions respond better to poliovirus RNA than to other viral RNAs. Even the less pure fractions make a specific copy of the added template, as shown by hybridization of the product to its template RNA but not to other RNAs. Among homopolymers only poly(A)-oligo(U) was copied by the replicase; other primed homopolymer templates were inactive.

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References

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