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. 2012;7(2):e31154.
doi: 10.1371/journal.pone.0031154. Epub 2012 Feb 3.

Protein solubility, digestibility and fractionation after germination of sorghum varieties

Abd El-Moneim M R Afify  1 , Hossam S El-Beltagi, Samiha M Abd El-Salam, Azza A Omran
Affiliations

Protein solubility, digestibility and fractionation after germination of sorghum varieties

Abd El-Moneim M R Afify et al. PLoS One. 2012.

Abstract

The changes in crude protein, free amino acids, amino acid composition, protein solubility, protein fractionation and protein digestibility after germination of sorghum were investigated. Sorghum varieties (Dorado, Shandaweel-6, Giza-15) were soaked for 20 h followed by germination for 72 h; the results revealed that crude protein and free amino acids in raw sorghum varieties ranged from 10.62 to 12.46% and 0.66 to 1.03 mg/g, respectively. Shandaweel-6 was the highest variety in crude protein and free amino acids content. After germination, crude protein was decreased and free amino acids were increased. There was an increase in content of valine and phenylalanine amino acids after germination. On the other hand, there was a decrease in most of amino acids after germination. After germination protein solubility was significantly increased. Regarding protein fractions, there was an increase in albumin, globulin and kafirin proteins and a decrease in cross linked kafirin and cross linked glutelin after germination.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Protein fractions of sorghum after germination (Alb: Albumin; Glo: Globulin; Kaf: Kafirin; Glu L Pro: Glutelins like protein; CL Kaf: Cross linked kafirins; CL Glu: Cross linked glutelins).
Treatments: (RD: Raw Dorado; RS: Raw Shandweel-6; RG: Raw Giza-15; GD: Germinated Dorado; GS: Germinated Shandaweel-6; GG: Germinated Giza-15).
Figure 2
Figure 2. In vitro protein digestibility (IVPD%) of sorghum after germination.
Treatments: (RD: Raw Dorado; RS: Raw Shandweel-6; RG: Raw Giza-15; GD: Germinated Dorado; GS: Germinated Shandaweel-6; GG: Germinated Giza-15).
See this image and copyright information in PMC

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