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Case Reports
. 2012:5:9-14.
doi: 10.2147/IJNRD.S27553. Epub 2011 Dec 29.

Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis

Yoshiyuki Morishita  1 , Minami Watanabe, Ichiro Hirahara, Tetsu Akimoto, Shigeaki Muto, Eiji Kusano
Affiliations
Case Reports

Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis

Yoshiyuki Morishita et al. Int J Nephrol Renovasc Dis. 2012.

Abstract

Purpose: Peritoneal dialysis (PD) is a successful renal replacement therapy; however, long-term PD leads to structural and functional peritoneal damage. Therefore, the monitoring and estimation of peritoneal function are important in PD patients. Oxidative stress has been implicated as one possible mechanism of peritoneal membrane damage. The aim of this study was to evaluate the association between an oxidative stress marker, 8-hydroxydeoxyguanosine (8-OHdG), and peritoneal damage in PD patients.

Methods: The authors evaluated 8-OHdG in drained dialysate by enzyme immunoassay to investigate the association between 8-OHdG and solute transport rate estimated by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in 45 samples from 28 PD patients.

Results: The 8-OHdG level was significantly correlated with dialysate:plasma creatine ratio (r = 0.463, P < 0.05) and significantly inversely correlated with D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection) (r = -0.474, P < 0.05). The 8-OHdG level was also significantly correlated with MMP-2 level (r = 0.551, P < 0.05), but it was not correlated with the age of subjects, the duration of PD, or blood pressure.

Conclusion: The level of 8-OHdG in drained dialysate may be a useful novel marker of peritoneal damage in PD.

Keywords: MMP-2; oxidative stress; peritoneal equilibration test; solute transport rate.

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Figures

Figure 1
Figure 1
The level of 8-hydroxydeoxyguanosine (8-OHdG) in drained dialysate in peritoneal dialysis (PD) patients. Comparison of 8-OHdG levels in drained dialysate between (A) males and females, (B) those who had diabetic nephropathy (DMN) and those who did not (non-DMN), and (C) those who had a past history of peritonitis and those who did not. Note: Columns represent the mean plus or minus standard error. Abbreviation: NS, no statistical significance.
Figure 2
Figure 2
Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal solute transport rate, determined by peritoneal equilibration test and matrix metalloproteinase-2 (MMP-2) level in drained dialysate: 8-OHdG level and (A) dialysate:plasma creatine ratio (D/P Cr), D/D0 glucose (where D is the glucose level of peritoneal effluents obtained 4 hours after the injection and D0 is the glucose level obtained immediately after the injection), and MMP-2 level in peritoneal dialysis (PD) patients and (B) D/P Cr, D/D0 glucose, and MMP-2 level in those PD patients who had a past history of peritonitis and in those PD patients who did not.
Figure 3
Figure 3
Correlation between the 8-hydroxydeoxyguanosine (8-OHdG) level in drained dialysate and the peritoneal dialysis (PD) duration and age of subject: 8-OHdG level and (A) PD duration and age of subject and (B) systolic blood pressure (SBP) and diastolic blood pressure (DBP).
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