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. 2012;130(1):37-43.
doi: 10.1590/s1516-31802012000100007.

Incidence of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in patients with urinary tract infection

Sobhan Ghafourian  1 , Zamberi Sekawi, Vasanthakumari Neela, Afra Khosravi, Mohammad Rahbar, Nourkhoda Sadeghifard
Affiliations

Incidence of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in patients with urinary tract infection

Sobhan Ghafourian et al. Sao Paulo Med J. 2012.

Abstract
in English, Portuguese

Context and objectives: Resistant bacteria are emerging worldwide as a threat to favorable outcomes from treating common infections in community and hospital settings. The present investigation was carried out to study the incidence of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae in patients with urinary tract infection in different seasons of the year, in order to determine the prevalence of the genes blaTEM, blaSHV and blaCTX-M, which are responsible for ESBL production among ESBL-producing K. pneumoniae, in three cities in Iran, and to investigate the antimicrobial susceptibility pattern of K. pneumoniae in different seasons.

Design and setting: Retrospective study carried out among patients with urinary tract infections in five hospitals in Iran.

Method: Two hundred and eighty-eight clinical isolates of K. pneumoniae were collected between March 2007 and April 2008 from five hospitals in three cities in Iran. ESBLs were identified by phenotypic and genotypic methods. ESBL-producing Klebsiella pneumoniae were evaluated against non-beta-lactam antibiotics. Genes coding for ESBLs (blaSHV, TEM and CTX-M) were screened.

Results: Among the 288 clinical isolates of K. pneumoniae, 37.7%, 46.7% and 15.6% were obtained from hospitals in Ilam, Tehran and Tabriz, respectively, of which 39.4%, 50.7% and 45.8% were ESBL-producing K. pneumoniae in Ilam, Milad and Emam Reza hospitals, respectively.

Conclusion: According to the results from this study, resistance to third-generation cephalosporins is higher during the cold months than during the warm months.

CONTEXTO E OBJETIVOS:: As bactérias resistentes estão surgindo em todo o mundo como uma ameaça ao resultado favorável no tratamento de infecções comuns em ambientes comunitários e hospitalares. O presente estudo foi realizado com o objetivo de avaliar a incidência de Klebsiella pneumoniae produtoras de beta-lactamases de espectro estendido (BLEEs) em pacientes com infecção do trato urinário em diferentes estações do ano, para verificar a prevalência dos genes blaTEM, blaSHV e blaCTX-M responsáveis para a produção de BLEEs emK. pneumoniae productoras de BLEEs, em três cidades do Irã, e investigar o perfil de sensibilidade antimicrobiana de K. pneumoniae em diferentes estações.

DESENHO E LOCAL:: Estudo retrospectivo realizado em pacientes com infecções do trato urinário em cinco hospitais no Irã.

MÉTODO:: Duzentos e oitenta e oito isolados clínicos de K. penumoniae foram coletados entre março de 2007 e abril de 2008 em cinco hospitais de três cidades no Irã. BLEEs foram identificados por métodos fenotípicos e genotípicos. K. pneumoniae produtoras de BLEEs foram avaliadas contra antibióticos não beta-lactâmicos. Os genes codificadores de BLEEs (blaSHV, TEM e CTX-M) foram investigados.

RESULTADOS:: Entre os 288 isolados clínicos de K. pneumoniae, 37,7%, 46,7% e 15,6% eram provenientes dos hospitais em Ilam, Tehran e Tabriz, respectivamente, dos quais 39,4%, 50,7% e 45,8% eram K. pneumoniae produtoras de BLEEs nos hospitais em Ilam, Milad e Eman Reza, respectivamente.

CONCLUSÃO:: De acordo com os resultados deste estudo, a resistência às cefalosporinas de terceira geração é maior nos meses frios do que nos meses quentes.

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Conflict of interest statement

Conflict of interest: None

Figures

Figure 1.
Figure 1.. Frequencies of blaSHV, blaTEM and blaCTX-M in different hospitals.
See this image and copyright information in PMC

References

    1. Chaudhary U, Aggarwal R. Extended spectrum -lactamases (ESBL) - an emerging threat to clinical therapeutics. Indian J Med Microbiol. 2004;22(2):75–80. - PubMed
    1. Knothe H, Shah P, Krcmery V, Antal M, Mitsuhashi S. Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection. 1983;11(6):315–317. - PubMed
    1. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001;14(4):933–951. table of contents. - PMC - PubMed
    1. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005;18(4):657–686. - PMC - PubMed
    1. Winokur PL, Brueggemann A, DeSalvo DL, et al. Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase. Antimicrob Agents Chemother. 2000;44(10):2777–2783. - PMC - PubMed

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