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. 1997 Nov;8(6):318-22.
doi: 10.1155/1997/828612.

Epidemiological investigation of Salmonella tilene by pulsed-field gel electrophoresis and polymerase chain reaction

C M Anand  1 , K Fonseca, K Longmore, R Rennie, L Chui, M Lingley, D Woodward
Affiliations

Epidemiological investigation of Salmonella tilene by pulsed-field gel electrophoresis and polymerase chain reaction

C M Anand et al. Can J Infect Dis. 1997 Nov.

Abstract
in English, French

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates of Salmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case of S tilene described in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes of Escherichia coli demonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.

L’électrophorèse sur gel à champ pulsé et des techniques d’empreintes génétiques (tests d’amplification génique) ont été appliquées sur onze isolats de Salmonella tilene : cinq souches provenaient de patients humains, six de phalangers du sucre et de hérissons nains gardés comme animaux de compagnie dans des familles ou dans des animaleries, et un isolat provenant du premier cas nord-américain de S. tilene décrit dans l’état Washington en 1994. L’électrophorèse sur gel à champ pulsé basée sur leur modèle de restriction a révélé que tous les isolats étaient semblables. Toutefois, les tests d’amplification génique à l’aide d’amorces des gènes de l’ARNr 16 et 23 d’Escherichia coli ont permis de démontrer que l’isolat de l’état de Washington différait des autres qui étaient tous semblables sur le plan de l’empreinte génétique de leur ADN. Cette étude indique qu’il est peut-être insuffisant de ne se fier qu’à une technique pour montrer les nuances entre des souches qui sont à plusieurs points de vue apparentées.

Keywords: Polymerase chain reaction; Pulsed-field gel electrophoresis; Salmonella tilene.

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Figures

Figure 1
Figure 1
Pulsed-field gel electrophoresis pattern of Xba1 digests of genomic DNA from Salmonella tilene isolates. Lanes 1 to 10: 22/77 (Washington State isolate), 44950, 44951, 44952, 93116, 93120, 97315, E2212, R763, R849 (see Table 1 for description of strains). Arrow indicates band with different migration rate
Figure 2
Figure 2
DNA fingerprint profile of Salmonella tilene isolates generated to conserved regions of Escherichia coli 16/23S rRNA by polymerase chain reaction. Lanes 1 to 11: 22/77 (Washington State isolate), 44950, 44951, 44952, 44209, 93116, 93120, 97315, E2212, R763, R849 (refer to Table 1 for description of strains). Lanes 12, 13, 14 and 15 are Salmonella johannesburg, Salmonella newport, negative control and 1 kb molecular weight marker
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