Electroanalytical sensors and devices for multiplexed detection of foodborne pathogen microorganisms

Abstract

The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered.

Keywords: electrochemical sensors; multiple detection; pathogen microorganisms.

Figure 1.

Schematic illustration of impedance multiplexed detection of rat IgG, HBsAg and HBeAg (Yu et al. [16]).

Figure 2.

(A) Electrical biochip microarray design and detailed zoom view. (B) Fully automated eBioChip Array analyzer “eMicroLISA” with fluidic, rotor valve, reagent reservoirs, and one hand plug and play ChipStick. Reproduced from Elshoz et al. [13].

Figure 3.

DOX-PCA concept [32]. (A–C) represent DOX responses for high, medium and low cell concentrations, respectively.